Journal: mBio

Article Title: Intestinal Microbiota Composition Modulates Choline Bioavailability from Diet and Accumulation of the Proatherogenic Metabolite Trimethylamine- N -Oxide

doi: 10.1128/mBio.02481-14

Figure Lengend Snippet: Bacterial strains used to colonize germ-free mice

Article Snippet: While E. tarda ATCC 23685 strain generated TMA from choline, ATCC 15947 strain did not, suggesting that the ability of microbes to convert choline to TMA is a strain-specific metabolic trait that may be acquired via lateral gene transfer.

Techniques: In Vitro

Journal: PLoS ONE

Article Title: High Expression of CD109 Antigen Regulates the Phenotype of Cancer Stem-Like Cells/Cancer-Initiating Cells in the Novel Epithelioid Sarcoma Cell Line ESX and Is Related to Poor Prognosis of Soft Tissue Sarcoma

doi: 10.1371/journal.pone.0084187

Figure Lengend Snippet: A. Expression of CD109 mRNA in ES cell lines. Bars represent mean±SEM. ※※※ p <0.001, determined by the Mann-Whitney test. N.S.: not significant. B. Expression of CD109 mRNA in human sarcoma cell lines. Cell lines of epithelioid sarcoma (FU-EPS-1 and VA-ES-BJ), osteosarcoma (OS2000, KIKU, NY, U2OS, Saos-2, HuO9 and HOS), Ewing sarcoma (SKES, WES and RDES), synovial sarcoma (Fuji and YaFuSS) and malignant fibrous histiocytoma (MFH2003 and MFH2004) were used. C. Expression of CD109 mRNA in human fetal tissues (upper panel) and human adult tissues (lower panel). ESX was used as a positive control. D. Immunohistochemistry of CD109 in normal adult tissues.

Article Snippet: Human osteosarcoma cell lines (NY, U2OS and HOS), human Ewing sarcoma cell lines (SKES, WES, and RDES), the human synovial sarcoma cell line FUJI, and the human ES cell line VA-ES-BJ were purchased from the Japanese Collection of Research Bioresources Cell Bank (Tokyo, Japan) and American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, MANN-WHITNEY, Positive Control, Immunohistochemistry

Journal: Journal for Immunotherapy of Cancer

Article Title: Analysis of tumor-infiltrating NK and T cells highlights IL-15 stimulation and TIGIT blockade as a combination immunotherapy strategy for soft tissue sarcomas

doi: 10.1136/jitc-2020-001355

Figure Lengend Snippet: Combined IL-15 stimulation and TIGIT blockade augments killing of sarcomas in vitro when target expression of TIGIT ligands is high. Analysis of the TCGA-SARC database by Kaplan-Meier analysis shows a significant survival advantage in tumors with a (A) high NK signature (p=0.0003), (B) high CD8 signature (p=0.03), but independent of (C) TIGIT expression (p=0.17). (D) TIGIT expression within STS tumors has a strong positive correlation with NK signature (r=0.82, p<0.0001) and CD8 signature (r=0.90, p<0.0001). (E) Low expression of the primary TIGIT ligand CD155 was associated with improved overall survival by Kaplan-Meier analysis of the TCGA-SARC database (p=0.04). (F) Expression of TIGIT ligand CD155 on sarcoma cell lines A673 (red) and SK-LMS (blue) compared with FMO staining. (G) Increased cytotoxicity of CFSE-labeled target cells A673 (left) and SK-LMS (right) following 4-hour incubation with in vitro IL-15-preactivated PBMCs from a patient with sarcoma in the presence anti-TIGIT 10 ug/mL (red) compared with IgG control 10 ug/mL (blue) at low and high E:T ratios. Increased expression of degranulation marker CD107a on NK cells from IL-15-preactivated PBMCs following 4-hour incubation with sarcoma cell lines shown by (H) representative flow cytometry against A673 cells, and (I) quantified per cent expression against A673 and SK-LMS cell lines in a 5:1 E:T ratio. These experiments were repeated independently in two patients with STS with representative data shown. P value determined using Student’s t-test using n=3–4 technical replicates. FMO, Fluorescence Minus One; IL, interleukin; PBMCs, peripheral blood mononuclear cells; STS, soft tissue sarcoma; TCGA, The Cancer Genome Atlas.

Article Snippet: Human sarcoma cell lines A673 (#CRL-1598, Ewing’s sarcoma) and SK-LMS (#HTB-88, leiomyosarcoma) were obtained from ATCC (Manassas, Virginia, USA) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% Nu-Serum and 1% penicillin-streptomycin.

Techniques: In Vitro, Expressing, Staining, Labeling, Incubation, Control, Marker, Flow Cytometry, Fluorescence